| Registro completo |
| Provedor de dados: |
Anais da ABC (AABC)
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| País: |
Brazil
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| Título: |
Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus
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| Autores: |
Alves,Gutemberg G.
Marinho-Carvalho,Monica M.
Atella,Georgia C.
Silva-Neto,Mario A.C.
Sola-Penna,Mauro
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| Data: |
2007-03-01
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| Ano: |
2007
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| Palavras-chave: |
Phosphofructokinase
Metabolism
Insect
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| Resumo: |
6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect which is usually a poor flyer, was measured and compared in two metabolically active tissues - flight muscle and fat body. The activity of this important regulatory glycolytic enzyme was much more pronounced in muscle (15.1 ± 1.4 U/mg) than in fat body extracts (3.6±0.4 U/mg), although the latter presented higher levels of enzyme per protein content, as measured by western-blotting. Muscle extracts are more responsible than fat body to ATP and fructose 6-phosphate, both substrates of PFK. Allosteric regulation exerted by different effectors such as ADP, AMP and fructose 2,6-phosphate presented a singular pattern for each tissue. Optimal pH (8.0-8.5) and sensitivity to pH variation was very similar, and citrate was unable to inhibit PFK activity in both extracts. Our results suggest the existence of a particular PFK activity for each tissue, with regulatory patterns that are consistent with their physiological roles.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100008
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| Editor: |
Academia Brasileira de Ciências
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| Relação: |
10.1590/S0001-37652007000100008
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| Formato: |
text/html
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| Fonte: |
Anais da Academia Brasileira de Ciências v.79 n.1 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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